Cyclic 3',5'-nucleotide phosphodiesterase; cytochemical localization in rat renomedullary interstitial cells.
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چکیده
منابع مشابه
Cyclic-nucleotide Phosphodiesterase
Cyclic nucleotides have been implicated in the differentiation and function of the vertebrate retina. In the normal retina of DBA mice, the specific activity of cyclic-nucleotide phosphodiesterase (PDE), with cyclic-AMP as the substrate (cAMP-PDE), increases eightfold between the 6th and 20th postnatal day. Kinetic analysis of retinae from newborn mice reveals a PDE with a single Michaelis cons...
متن کاملIsoelectric-focusing patterns of cyclic nucleotide phosphodiesterase from rat heart.
1. Isoelectric focusing on a flat gel bed of the rat heart cytosolic fraction resolved cyclic nucleotide phosphodiesterase activity into several forms, characterized by their substrate specificity, kinetic constants and dependence towards Ca2+ and calmodulin. A peak of pI 4.9 displayed 20 times more affinity for cyclic GMP than for cyclic AMP and was markedly inhibited by EGTA. A less substrate...
متن کامل3'-cyclic nucleotide 3'-phosphodiesterase
1. A spectrophotometric assay of 2':3'-cyclic nucleotide 3'-phosphodiesterase (EC 3.1.4.37) based on the use of an acid-base indicator and a buffer having identical PKa values is described. The assay is sinmple and rapid; it was particularly convenient for monitoring the enzyme activity at vat :ous stages of purification. 2. Several proteinases were examined for their ability to solubilize 2':3...
متن کاملCyclic nucleotide phosphodiesterase activity in normal and neoplastic rat mammary cells grown in monolayer culture.
The activity of cyclic 3':5'-nucleotide phosphodiesterase (PDE) (EC 3.1.4.17) was measured in cultured normal and neoplastic rat mammary epithelium. Total PDE activity in normal cells was 1.6 to 6 times higher than that in tumor cells over a concentration range of 0.01 to 1 mM cyclic adenosine 3':5'-monophosphate. PDE activity was distributed between the low-speed (4000 x g) particulate and sup...
متن کاملCyclic nucleotide phosphodiesterase activities in Neurospora crassa.
Cyclic nucleotide phosphodiesterase activities in soluble Neurospora crassa mycelial extracts were resolved into two peaks, phosphodiesterase I and II, by chromatography on DEAE-cellulose columns. Phosphodiesterase I hydrolysed cyclic AMP and cyclic GMP equally well. Phosphodiesterase II was active on cyclic GMP but scarcely active on cyclic AMP. Phosphodiesterase I was resolved by gel filtrati...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1978
ISSN: 0022-1554,1551-5044
DOI: 10.1177/26.6.209105